Application of MED64 planar microelectrode array recording system in the study of brain neural activity in patients with epilepsy

      Epilepsy is a disease and syndrome characterized by intermittent central nervous system dysfunction caused by repeated and sudden over-discharge of brain neurons. It is a motor sensation, autonomic nerve, consciousness and recurrence that originated in the brain. Mental disorders of varying degrees. The incidence of epilepsy is wide, the effect of drug treatment is easy to repeat, and the risk of surgical treatment is high. Researchers have long been committed to the improvement and innovation of epilepsy treatment strategies.

NMDA receptors (N-methyl-D-aspartate receptors, NMDARs) within the central nervous system is an important class of excitatory amino acid (excitatory aminoacid, EAA) receptors. NMDA receptors not only play important physiological roles in the development of the nervous system, such as regulating neuronal survival, regulating neuronal dendrites, axon structure development and involvement in synaptic plasticity formation; the NMDA receptor also plays a key role, our data show that NMDA receptors in learning and memory in a class of receptors that are crucial. At present, the role of NMDARs in the process of epileptic seizures has been extensively studied, but little is known about the respective roles of two different NR2 subunits of NMDARs . A better understanding of the unique role of different subtypes of NMDARs in the development and progression of epilepsy can help develop more targeted strategies for the treatment of epilepsy.

Researchers Berretta N, Ledonne A, and others from the Neuroscience Laboratory in Italy conducted an in-depth study of brain neural activity in a rat model of epilepsy. In the latest issue of " Epilepsia " (Epilepsy), an article entitled " Hippocampus versus entorhinal cortex decoupling by an NR2 subunit-specific block of NMDA receptors in a rat in vitro model of temporal lobe epilepsy " was published. In this study, they used the MED64 system (Multi-Electrode Array System, Alpha MED Scientific Inc. Osaka . Japan ) Conducting electrophysiological studies. Will contain the entorhinal cortex (entorhinal cortex, EC) and the hippocampus brain slices placed on the electrode, with the addition of 4 - aminopyridine (4-aminopyridine, 4AP, 10-15 μ m) of Mg (2+) - The free medium was continuously perfused for incubation. Seizure-like epileptic activity in two independent rhythm patterns was induced in the EC and hippocampus. At the time of seizures, both activities occurred simultaneously in CA3 , showing a significant interrelated association. Incubation NR2A subunits of NMDAR antagonist antagonist [(R) - [(S ) -1- (4-bromo-phenyl) -ethylamino] - (2,3-dioxo-1,2,3,4-tetrahydroquinoxalin-5 -yl)-methyl]-phosphonic acid (NVP-AAM077; 50nm) or Zn(2+) (200nm) does not affect epileptic activity in the episodes of EC and hippocampus, but the correlation between the two is significant reduce. The above phenomenon was also observed when ( α R, β S) -α -(4-hydroxyphenyl) -β- methyl-4-(phenylmethyl)-1-piperidinepropanol maleate (Ro25-6981; 1 μm ) was incubated .

The results of the study indicate that during epileptic seizures, the NR2 subunit plays an important role in cortical neural network association activities, leading to more extensive neurological activity. Therefore, drugs targeting the NR2 subunit will help reduce the spread and association of neural network concussion in the brain of patients with epilepsy and alleviate the occurrence of the disease.

MED64 system (Planar Microelectrode Array Recording System, Alpha MED Scientific Inc.. Osaka . Japan ) is a complete and easy-to-use multi-channel isolated extracellular neurophysiological research tool. The system includes a complete set of hardware equipment consumables and analysis software required for in vitro electrophysiological studies. The user can conveniently and quickly perform electrophysiological examination on ex vivo materials such as brain slices, myocardial sections, cultured nerve cells or cardiomyocytes, stem cells, etc. The cells or tissues to be cultured directly on the electrode coating material can be allowed to measure. Local electrical changes directly adjacent to the cell, while simultaneously recording the electrophysiological signals of multiple cells. MED64 devices can simultaneously record and stimulate different sites; moreover, MED64 records are non-invasive and therefore do not damage cells. This feature makes them suitable for long-term recording and repeated recording on the same culture sample, thus allowing The longer-term evolution of the response of the culture to drug stimulation was monitored. Combined with the optical recording method, photoelectric combined detection can be realized.

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