Nursery facilities and water conditions 1. Nursery site: Located in the Marine Economic Development Zone of Ji County, Jiangsu Province, the infrastructure for breeding seawater seedlings includes power-pumping equipment, sedimentation and filtration systems, water and electrical systems, and daily management tools (replacement pipes, suction bottoms, and inverted pools). Box, etc.) The facilities are basically complete. The nursery water body is 2,600 cubic meters and is divided into a number of breeding ponds, each 25 cubic meters. 2. Seawater source: in line with national fishery water quality standards, nursery water is precipitated by a secondary filtration system, then filtered through a 300-mesh nylon sieve basket in the inlet of the pool, and no large-scale protozoa enter the pool after microscopic examination. Algae selection and bait cultivation 1. The species of unicellular algae: (1) Golden algae: Isochrysis galbana, Forsythium alginate; (2) Diatoms: Nitzschia closterium, Phaeodactylum tricornutum, Chaetoceros muelleri; (3) Green algae: Platymonas, Cynophyta, Chlorella. 2. Feed culture: (1) Grade 1 bottle algae culture: Use Conway solution as nutrient salt and boiling water; (2) Secondary relay culture: use polyethylene transparent film bag (cover net cage) to transition; (3) ) Three-level open culture: production is conducted in a dedicated bait culture pond to meet the requirements of the bait after the dear one enters the pond. Burley indoor artificial seedlings 1. Selection criteria for intimacy: Select burrows from 3rd to 4th age in natural sea areas and shells with a length of 7 cm or more as a brood, and require strong, non-invasive, less shedded, no parasites and diseases. Intimate specifications average 0.20 kg / only -0.25 kg / only, the purchase amount of 100 kg, the pool time for the June 10 that year. 2. Intimate the cooked method: Wash the shell surface dirt before entering the pool and place it in a floating cage (size 50 cm, 40 cm, 30 cm) for holding and promoting. Packing density is controlled at 20/cubic meters. The proportion of males and females is 1:1, and the quantity is controlled by 2 water/3 cubic meters/cubic meter. After entering the pool, the pro-environment was heated to 1°C per day and gradually raised to a constant temperature of 21°C. During this period, regular 2/3 change of water every day, depending on the water quality situation, the pool will be poured in due course. Feeding diets were mainly Nitzschia closterium and Phaeodactylum tricornutum, fed once every 3 hours, and each feeding amount was 30,000 cells/ml-80,000 cells/ml. During the pro-monetary period, continuous micro-inflation, shading, and regular detection of water quality. After 7 days normal temperature, cooked on June 17th, late 20:00-0:00, water temperature 23 °C, pro-spawning natural spawning. 3. Egg-laying hatching and larval rearing: (1) Spawning: The pro-pipa discharge time can be as long as ten minutes. During this time, the male foal is to be fished out. At the same time, the eggs are piled up by manual stirring to control the spawning density. 50 capsules/ml. At this point remove the intimidating female body to continue production, while sucking out the upper 1/2 of the fresh water, adding fresh filtered seawater. Repeated egg washing 2 times -3 times, antibiotic 2 g/m3, EDTA 2 g/m3. During the incubation, inflate for 10 minutes to agitate the pool water and remove the sticky floating foam. Stir the hatching pool once every hour after stopping the inflation to prevent the sinking of the fertilized eggs. (2) Incubation: Fertilized eggs hatch to D-type larvae at water temperature of 25°C in about 20 hours, and they are concentrated in the upper layer of the water body. At this time, the recovery of micro-aeration is started. The water level difference is controlled within 0.5m. If the larvae prolong the metamorphosis, there will be deformities. More than 30%, that is eliminated. The D-type larvae were selected and bred after 2 hours of complete metamorphosis, and the upper quality D-type larvae were transferred to a cultivation pond via a 350-mesh screen. The selected D type larvae were cultured at a density of 10 cells per milliliter. The amount of feeding was forked chlorella 20,000 cells/ml -3 million cells/ml or Chlorella 50,000 cells/ml -10 million cells/ml or Pyracantha 0.5 million cells/ml -2 million cells/ml, Mixed feeding is scaled down. The number of feedings was 3 times/day - 4 times/day, the amount of water changed once a day, and the pool was poured once every 5 days. The water change cage can be changed according to the growth status of D-type larvae, not 1 network day (300 mesh, 260 mesh, 200 mesh). Appropriate environmental conditions during larval rearing: water temperature 23°C-25°C, room temperature 28°C-30°C, salinity 26 ‰-34 ‰, diffuse light, DO ≥ 5 mg/L, COD ≤ 1 mg/L, pH Values ​​8.0-8.3, NH3-N ≤ 200 ppb. The larvae at the head of the shell can reach 120 μm and the average daily growth rate is more than 10 μm. 4. Feeding of attachments and nurturing of juveniles: (1) Putting in attachments: When about 30% of larvae have black spots and the shell length is between 250μm and 270μm, the polyethylene mesh sheet is placed at the appropriate time as the attachment base. The amount is 50 tablets. / Cubic meters (soaked in hydrochloric acid solution for 1 day - 2 days before use, washed with filtered seawater, dried). (2) Nursing of juveniles: After 5 days to 7 days, the attachment of eye-point larvae was completed, the metamorphosis rate was 40%-50%, the body length of larvae was 0.4μm-0.5μm, and the feed amount of bait was increased to 80,000 cells/ml-10. Million cells/ml while increasing the amount of water change. After the larvae were attached, they were changed to water culture and the water was changed twice a day. By July 15th, after 20 days and 25 days of cultivation, the average shell length of juveniles reached 1 mm. This artificial breeding experiment produced 2.3 million jumbo juveniles. The juvenile before going out of the pool was stimulated by running water for 24 hours to 56 hours with a flow rate of 20 cm/sec to 40 cm/sec. The purpose was to reduce the shedding rate of juveniles after going to the sea. The expulsion rate of juveniles stimulated by running water is 20%, and the expulsion rate without flowing water stimulation is above 30%. Cultivated in the middle of the burrowing sea area In the near sea area of ​​Haizhou Bay, the water depth is 3m to 4m, the water quality is fresh, the shelter is small, and the biological food is abundant. On July 15th, 170 million juveniles were moved to cultivate the sea area to protect the seedlings. The seedling bag is double-layered, the inner bag is on the 30th, the outer bag is on the 60th, and the specification is 25cm and 40cm. Each bag contains 10,000 -20000 grainy juveniles, and each 10 bags are single-stranded pendant stone. In the middle of the floating area on the sea. The bottom bag is 2 meters away from the seabed, and the string spacing is 1 meter. Brush the mesh bag once every 5 days. When the bag is reached 2 milliliters, it is time-divided into bags of 1500-2000 tablets per bag; the juveniles go to the sea for about 40 days, and when the shell of the seedlings is 0.5 centimeters long, the bags are divided into 20 mesh bags (single layer). Bags 500-1000 tablets; to mid-September larvae shells up to 0.9 cm-1 cm also timely split seedlings, that is, the use of bay scallop cage breeding burrowing juveniles, each layer of 100 -200; in early October When juveniles were up to 2 cm in size, they were trained in the middle of the sea area. A total of 51 million seedlings were planted and the survival rate was 30%. Burrowing 1. Proliferation and release of sea area: According to the background survey of the sea area, the 15m isopold line near the southeastern part of the sea area of ​​Haizhou Bay is designated as a burrow ploughing and breeding area. (Recent data shows that in 2009 there were burrowing resources in the sea area, when the catch amounted to 130 tons of fresh shellfish), there was no industrial pollution and freshwater impact in the protected area, the water depth was 11m to 15m, and the bottom water temperature was 22°C-24.5°C in August. , Salinity is stable at 29‰-32‰, dissolved oxygen saturation is 85%, sediment is fine clay (90μm) content above 80% soft mud bottom, sediment sulfur content is 0.1 mg/g(dry weight), organic matter consumption Oxygen 8 mg/g (dry weight). 2. Bottom sowing proliferation and release method: In mid-March, the sea area will enter the sea area for implementation of the broadcast. The method of investment seedlings is mainly based on underwater sowing, that is, the submersible workers and the people on board are working at the same time, and the seedlings are evenly spread on the bottom of the sea, with a sowing density of 15 grains per square meter. 3. Playback management at the bottom: The factors affecting the survival rate of burrowing ploughs are various, such as changes in salinity, changes in temperature, changes in COD, etc., and the most important influencing factors are the enemies of starfish and other harmful organisms. Invade. The main management method at present is to catch fish nets and set starfish trapping net bags on a regular basis. Shelled shrimps,Dried shrimps,Frozen Bamboo shrimp,Pandalus borealis,Red Shrimp,Coldwater Shrimp Zhejiang ocean family co.,ltd , https://www.ocean-family.com