China University of Science and Technology progresses in the study of animal long-chain non-coding RNA January 16, 2019 Source: University of Science and Technology of China The research team on non-coding RNA function and functional mechanism of the School of Life Sciences, University of Science and Technology of China recently published an article entitled Systematic evaluation of C. elegans lincRNAs with CRISPR knockout mutants in the international journal Genome Biology. The latest research results of the room. The laboratory systematically studied the long-chain non-coding RNA (lincRNA) of 155 genes in C. elegans by optimized gene editing technology, and systematically studied the nematodes of C. elegans. The function of lincRNA. This is the first study to use a gene editing technique in multicellular animals to knock out a long non-coding RNA at a genome-wide level and systematically analyze its physiological functions and functional mechanisms in animals. They screened six phenotypes of 155 lincRNA knockout mutants and found that 23 lincRNA mutants had different degrees of defects in one or both of their physiological phenotypes. By transcriptome sequencing of different developmental stages of C. elegans, the co-expression of lincRNA and mRNA was studied, and the co-expression and regulation network of lincRNA and microRNA were established. Through the analysis of nearly 300 transcription factors in different developmental stages of C. elegans to explore the regulation of lincRNA expression in different developmental stages of the transcription factor, the mechanism of these 23 lincRNAs to exercise their physiological regulation function was studied. This study systematically explored the physiological functions of lincRNA in multicellular animals and broadened the field of lincRNA research to some extent. At the same time, the lincRNA knockout nematode strain obtained in this study provides strong support for the further study of the role of long-chain non-coding RNA in aging and disease. The co-first authors of the article are doctoral student Wei Shuai, postdoctoral Chen He and recent international student Emmanuel Enoch Dzakah. The author of the communication is a single professor of the Chinese University of Science and Technology. The research was supported by the Ministry of Science and Technology, the National Fund Committee, and the Chinese Academy of Sciences' “Technology and Intervention Strategies for Agingâ€. China University of Science and Technology progresses in the study of animal long-chain non-coding RNA Children X Ray Lead Collar,Children Lead X-Ray Collar,Children X-Ray Protective Collar,Children Large Shaped X-Ray Lead Collar Longkou Kangxie Medical Instrument Co., Ltd , https://www.sdkangxiemedical.com