Comparison of NMT and laser confocal technology What is laser confocal Laser scanning confocal microscopy (LSCM) is a state-of-the-art molecular cell biology analytical instrument that uses computer, laser and image processing techniques to obtain three-dimensional data from biological samples. It is mainly used to observe the biological cell structure and biological changes of specific molecules and ions , quantitative analysis, and real-time quantitative determination. It not only can obtain very clear fluorescence images, but also locate and quantify multiple fluorescent markers. It also has functions such as image 3D reconstruction, fluorescence resonance energy transfer spectroscopy and even membrane potential measurement, which has become an important technical means for life science research . Limitations of laser confocal With the expansion of the application of laser confocal technology, its limitations in research have also become increasingly prominent. Laser confocal technology mainly collects ion molecular information inside biological samples . The changes of these ion molecular information may originate from the changes of ion/molecular source inside the sample, or may be caused by ion/molecular exchange inside and outside the sample. These two ion/molecular changes are triggered by completely different life mechanisms. This requires researchers to pass other experimental results in order to draw relatively accurate conclusions . If laser confocal data is used as a test or diagnostic standard, it often faces a large risk of false positives . NMT contrast laser confocal Commonality real time dynamic data visualization Determination of free ions difference NMT can measure a wide variety of samples NMT can measure two ions simultaneously Combine The combination of laser confocal technology and non-damage micro-measurement technology ( NMT ) can overcome the risk of false positives in laser confocal data as a detection or diagnostic standard, and achieve comprehensive ion/molecular flow inside and outside the sample. information. Realize both internal and external testing . Greenhouse Vertical Hydroponics
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Laser confocal technology Non-damage micro-testing technology Use dyes and laser sources Use electrodes or sensors Need to mark No need to mark Fluorescence is prone to quenching Electrode or sensor stabilization Short measurement time Measurement time can be short and can be long Semi-living (with damage) Approximate living or complete living (measured without damage) Detect internal ion concentration changes Detect ion flow rate across the membrane and external ion concentration Less type of determination, depending on the dye There are many kinds of determination, and can measure N a + , K + , N O 3 - , O 2 , etc. Measuring materials should not be too large, mainly cells Measurement materials are not limited, they can be measured from cell to whole Only one ion can be measured simultaneously Simultaneous determination of two ions 
