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Comparison of the operation of automatic gel dyeing workstation and traditional bleaching shaker
Comparison of the operation of automatic gel dyeing workstation and traditional bleaching shaker
Since its launch in mid-2012, Dingyuanyuan gel dyeing workstation has received extensive attention from universities, research institutes and biotechnology companies. Good results have been achieved in the trials and purchases of universities and research institutions across the country, including:
China Academy of Agricultural Sciences, Liaoning Academy of Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Tianjin Academy of Agricultural Sciences, Chinese Academy of Sciences Oil Institute, China Agricultural University, Huazhong Agricultural University, South China Agricultural University and many other units.
The DHS gel staining workstation was originally designed to automate the silver nitrate staining process after SDS-PAGE electrophoresis, replacing the experimental operation on a traditional bleaching shaker. Through the microcomputer-controlled program, the DHS gel dyeing workstation realizes unattended operation in the silver dyeing process, eliminating the time of manual liquid addition, liquid exchange and rinsing, greatly improving the daily work efficiency of the laboratory, and improving the experiment through program operation. Repeatability. At the same time, the contact time with the toxic reagent is obviously reduced, and the physical damage to the experimenter is reduced. The reagents circulate in a relatively closed environment and the user does not have to worry about contamination in the laboratory environment. Make up for the potential concerns of using a bleaching shaker.
From the hardware point of view, the workstation uses a separate 11-channel liquid/discharge line, which reliably avoids cross-contamination of reagents and achieves reagent recovery after the experiment. The dyeing tank of the workstation oscillates in the same way as the shaker and is equipped with a cover to prevent the coloring liquid from evaporating. In terms of software, the simple and clear operation interface allows the user to freely set the inlet and outlet lines, the amount of fluid, and the shaking time.
Of course, the user can set the workstation program according to the normal experimental procedure to achieve the experimental expectation, but in the process of silver nitrate gel staining, sometimes the color rendering process is difficult to master the time, then how to achieve manual intervention with the automated program? The dyeing chamber of the DHS gel staining station is made of dark transparent material. Users can observe the strip directly during the color development process. You can choose to terminate or skip the next step in any step to avoid excessive gel staining. In addition, the dark dyeing tank eliminates the effect of sunlight on the silver nitrate solution. DHS dyeing workstations can easily handle a variety of common experimental requirements during our actual use with our users.
The dyeing effect is shown in the figure below, which is equivalent to the traditional bleaching shaker dyeing:
In addition, the effect of the automated gel staining workstation is also excellent in other staining methods such as dyeing on SDS-PAGE.