Hybridoma positive clone enhanced adjuvant use method

Hybridoma positive clone enhanced adjuvant

Positive Clone Stimulate Adjuvant

The cell electrofusion method gradually replaces the polyethylene glycol method (PEG) with its unique advantages such as no chemical toxicity, standardization of operation, and high cloning rate, and has become a mainstream hybridoma cell production method. In the same case, the number of fusion clones produced by electrofusion is 5-10 times that of PEG. But the fly in the ointment is that the positive rate of clones produced by this method will not be improved.

Hybridoma-positive clone-enhanced adjuvants from Qiwen Bio are the only adjuvant products in the world that can increase the positive rate of cloning. After multiple rounds of immunization of the animal, the adjuvant is activated by stimulation, increasing the number of B cells by 2 times. If used in conjunction with a cell electrofusion apparatus, the positive rate can be increased by 5-10 times while increasing the clone yield by 5-10 fold.

Instructions:
1. Traditional methods of normal immunity;
2. After 30 minutes of Pre-fusion boost injection of the antigen, the product was intraperitoneally injected with 100 μl/mouse; after 3-4 days, the spleen or lymph node was taken for fusion.

Effect:

Product specifications:

Item number

specification

Dosage

PCA001

1ml

100μl each time, a total of 10 times

Storage Conditions:

2 - 8 ° C, 6 months; -80 ° C, 2 years.

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