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purpose
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This article will demonstrate the use of a CORTECS UPLC column to separate five structurally similar local anesthetics (Figure 1), resulting in an improvement compared to the initial separation of a fully porous column. 
Improve resolution with CORTECS UPLC columns
Kenneth Berthelette, Mia Summers and Kenneth J. Fountain
Verifies the high separation performance of CORTECSTM UPLC ® 1.6 μm columns.
For complex mixture separations, high resolution is an important quality indicator, and the accuracy of the calculation can be improved by reducing the interference of similar elution peaks. The resolution is a function of system efficiency (N), selectivity between compounds (α), and retention factor (k). Since the square root of the column separation efficiency, and therefore high column efficiency for the column, which is a higher degree of separation. The use of a highly efficient CORTECS UPLC 1.6 μm column allows for better analyte separation without further optimization of the analytical method.
Local anesthetics were separated using hydrophilic interaction chromatography (HILIC) to demonstrate the high separation capacity of the CORTECS UPLC column for different peaks. Local anesthetics are widely used in many medical fields, including dentistry.
Efficient CORTECS UPLC columns increase the resolution of difficult-to-separate materials for more accurate characterization and quantification of analytes.
In order to accurately quantify or characterize peaks in liquid chromatography, it is important to minimize the potential interference of similar elution peaks. The more theoretical plates the column has, the higher the column efficiency and the higher the resolution. Waters ® CORTECS UPLC high-performance columns feature advanced solid core particle and column packing technology for high resolution. An example of local anesthetic separation (Figure 2) is the comparison of the initial separation of the fully porous HILIC column with the separation of the CORTECS HILIC UPLC column, using exactly the same conditions.
In this study, the original column was replaced with a CORTECS UPLC HILIC column, and the resolution between peak 2 and peak 3 increased by 51%. In the initial separation, peak 2 and peak 3 were significantly co-eluting with a resolution of 1.18. When using the CORTECS UPLC HILIC column, baseline separation was achieved with a resolution of 2.15, enabling accurate characterization or quantification of tetracaine (2) and bupivacaine (3). Because the CORTECS UPLC column uses the same method conditions as the initial separation, no further development methods are required, increasing laboratory resource utilization efficiency.
to sum up
It is very difficult to obtain high peak-to-peak resolution in difficult isolates. Method development for improved resolution may require more time, resulting in reduced work efficiency; the use of high-efficiency CORTECS UPLC columns improves the resolution of difficult-to-separate analytes for more accurate characterization and quantification of analytes .
references
1. Neue UD. HPLC Columns: Theory, Technology, and Practice. Wiley-VCH. 1997.