AAV's "birth", they are enough! In daily life, we often hear or personally participate in various "circles", such as fashion circles, entertainment circles, art circles, as well as Shanghai's major business districts and edible donuts. Of course, right Most people are most familiar with WeChat friends. In other words, there is also a circle in the field of gene therapy. Xiaobian temporarily named it "viral carrier circle". There is such a star-studded star in this circle. It is not only unique in physical and chemical properties (it can withstand freezing and thawing, 65 ° C high temperature and a variety of organic solvents), and with stunts, while having a strong plasticity (divided into 12 serotypes and more than 100 mutants), is widely favored by researchers and medical workers. In today's life sciences, the pursuit of it seems to have become a common practice, who is the magic rookie of this "viral carrier circle"? AAV was originally named as a contaminating component found in purified adenovirus fluids. Its replication and proliferation requires the assistance of a helper virus such as adenovirus or herpes virus, so AAV is a replication-defective virus belonging to the genus of the genus Parvoviral. AAV is a non-enveloped single-stranded linear DNA virus with a genome of about 4.7 kb and an inverted terminal repeat sequence (ITR) at both ends. The ITR sequence is a viral protein coding region, which contains two open The reading frame produces the replication protein Rep, the capsid protein Cap, and the packaging activator protein AAP. As the earliest FDA-approved gene therapy vector, AAV has been widely used because of its wide host range, non-pathogenicity, low immunogenicity, long-term stable expression of foreign genes, good diffusion performance and stable physical properties. It is used in basic research and clinical trials. Moreover, more and more clinical trials have proved the effectiveness and good safety of AAV vectors, making it a hot spot in gene therapy research and considered to be one of the ideal carriers for gene therapy. So, where does AAV, which is so popular and has its own aura of stars, come from? In this issue, Xiaobian will bring you the "A birth" of AAV. In the viral gene delivery system, there is a big concern that the helper plasmid and the vector plasmid are recombined to restore the wild type virus. Most current protocols for generating recombinant adeno-associated virus (rAAV) require co-transfection of a vector plasmid and a packaging plasmid expressing viral replication and structural genes into adenovirus (Ad) infected culture cells. This undoubtedly increases the likelihood of wild-type AAV production. Jike's AAV production is enough for plasmids and cells! Our "AAV Helper-Free System" consists of three plasmids: viral vector, pAAV-RC vector and pHelper vector. Among them, the pAAV-RC vector plasmid contains the rep and cap genes of AAV-encoding replication protein and viral capsid protein; the pHelper vector plasmid provides the adenoviral gene products required for the production of infectious AAV virus particles (for example: E2A, E4) And the VA RNA gene); the remaining adenoviral gene products are provided by host cells stably expressing the adenovirus E1 gene. The biggest advantage of the packaging system is that it does not need to add a helper virus. On the one hand, it eliminates the contamination of rAAV by the helper virus; on the other hand, it reduces the possibility of wild-type AAV production and further improves the safety of AAV products. The AAV products produced by Jikai are able to guarantee the quality and yield of the rAAV gene vector required for animal testing. If you are a research fan who is passionate about AAV research, if you and your research partners are still worried about which AAV to choose? Don't hesitate any more, welcome to pay attention to the WeChat public account above. Jikai can answer your questions for why you are 10,000! We have a professional research team and quality technical services, and we sincerely invite you to cooperate! Hair Wash Shampoo,Natural Shampoo,Hair Nourishing Shampoo,Ginger Hair Shampoo Wuxi Keni Daily Cosmetics Co.,Ltd , https://www.kenicosmetics.com
It is Adeno-Associated Viral Vector (AAV). 
Figure 1 Structure of the AAV genome 
Figure 2 AAV production schematic (original) 
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