Gelred is an excellent fluorescent nucleic acid gel staining reagent that is highly sensitive, low toxicity and ultra stable. It has wide adaptability and simple dyeing process. The following is a brief introduction to the use of gelred 1. Glue method (usage with EB) (1) Add GelRed nucleic acid dye when making the gel (for example, add 5 μL of GelRed 10,000× stock solution per 50 mL of agarose solution, in analogy). (2) Electrophoresis was carried out in accordance with a conventional method. Precautions: This method uses relatively little dye dye. Approximately 50 pieces of 50 mL of glue can be made with 500 μL of dye. Because GelRed has good thermal stability, it can be added directly in hot agarose solution without waiting for the solution to cool. Shake, shake or flip to ensure the dye is fully mixed. Alternatively, the GelRed stock solution can be added to the agarose powder and running buffer and then heated in a microwave oven or other conventional manner to prepare an agarose gel. GelRed is compatible with all common electrophoresis buffer solutions. Pre-formed gel solutions containing dyes can be prepared in large quantities and stored for long periods of time until use. This method is not suitable for pre-formed polyacrylamide gels. For polyacrylamide gels, use bubble dyeing. 2. Bubble dyeing (1) Electrophoresis was carried out in accordance with a conventional method. (2) The GelRed 10,000× stock solution was diluted with H2O by about 3,300 times to 0.1 M NaCl to prepare a 3× staining solution. (For example, 15 μL of GelRed 10,000× stock solution and 5 mL of 1 M NaCl were added to 45 mL of H 2 O). (3) Carefully place the gel in a suitable container, such as a polypropylene container. Slowly add a sufficient amount of 3X staining solution to immerse the gel. The dyeing was performed at room temperature for about 30 minutes, and the optimal staining time was slightly different depending on the gel thickness and the agarose concentration. For gels containing 3.5 to 10% acrylamide, the dyeing time is usually between 30 min and 1 h and is prolonged as the acrylamide content increases. Precautions: When dyeing by bubble dyeing, the amount of dye is more. The single-use staining solution can be reused about 3 times. The 3× GelRed staining solution can be prepared in large quantities and stored in the dark at room temperature until it is used up. Light Color Quarters Walnuts Kernels 1/4 Walnut Kernels , light color, broken while hand shelling .size is equal to or bigger than 1/4 walnut kernels, Light Color Quarters Walnuts Kernels,Light Color Walnuts Kernels,Hot Walnut Kernels Light,Walnut Kernels Light Quarters Weishan Yuanxing Walnuts Co.,Ltd. , https://www.walnutsxxy.com