Release date: 2014-11-25 Flowers bloom, another year, it is time to take stock of new changes in the field of next-generation sequencing. Since February of this year, the sequencer has not changed much, but it does not mean that everything is the same. The new sample preparation instrument is unveiling the veil, and the sequencing read length is even worse. Roche 454 454 Life Sciences announced in 2013 that it plans to stop sales of sequencing hardware in 2015. According to Susan Ulanowicz, a sequencing sales manager for Roche Diagnostics, the company will support its hardware until the end of 2016, rather than the mid-2016 that was previously reported. However, Roche NimbleGen is still developing NGS sample preparation tools. In January, it launched the SeqCap Epi sequence enrichment system to capture methylated genomic DNA. Recently, NimbleGen introduced the SeqCap RNA enrichment system, which uses oligonucleotides to enrich a specific portion of the transcriptome. According to Ji Wu, director of international marketing at Roche NimbleGen, there are three versions of the SeqCap RNA enrichment system: lncRNA design captures 17.35 Mb, representing 32,808 lncRNA and TUCP tran isomers; and two customized versions, from low to low A sequence of 7 Mb to 200 Mb was captured in 10 ng of total RNA. "It reduces the cost of sequencing and increases the ability to discover and identify novel exons, splice variants, mutations and gene fusions," Wu said. Illumina Joel Fellis, product marketing manager for sequencing systems at Illumina, said that Illumina has some updates to sequencing reagents this year. The HiSeq Rapid v2 Reagent Kit, released this month for the HiSeq 2500, will increase the read length in fast mode to 2 x 250 bp (dual end) and deliver 300 Gb in 60 hours. The HiSeq X HD v2 Reagent Kit will also be released in November. It allows researchers to prepare sequencing libraries faster without PCR amplification. “For the best genomic assembly, we recommend the TruSeq DNA PCR-Free kit because it brings excellent coverage in challenging areas, such as those with high GC content,†Fellis said. In July, Illumina unveiled the TruSeq Synthetic Long-Read DNA Library Prep Kit, a commercial product of the Meleculo technology acquired by Illumina two years ago, which was previously only available as a service. Fellis explained that the kit generates long-read data from Illumina short-reading technology by calculation. This information will aid in phasing and de novo assembly. The NextSeq 500 is a new product launched by Illumina this year. Fellis said that reagent upgrades will be released in the first half of next year. At the recent meeting of the American Society of Human Genetics, scientists demonstrated the accuracy of the new reagents for bringing the HiSeq level to the NextSeq 500. “You will get more accurate data than the previous reagents,†he said. Ion Torrent Ion Torrent's Ion PGM and Ion Proton instruments are now part of Thermo Fisher's, and there have been some reagent upgrades recently. In the third quarter, Thermo Fisher released a new Hi-QTM DNA polymerase, which is expected to significantly reduce the error rate of insertion loss. According to Andy Felton, product manager at Ion Torrent, for Staphylococcus aureus, the rate of insertion loss decreased by 94%, and E. coli decreased by 80%. For gene panels, this depends on the situation. Felton referred to a resequencing panel containing 2300 PCR amplicons, with an insertion loss rate of approximately 43%. Currently, Hi-Q for Ion PGM has been launched, and the formulation of Ion Proton should be launched in the first half of 2015. Another new product is the Ion AmpliSeq Transcriptome Human Gene Expression Kit, a PCR-based sequence enrichment tool that captures and sequences amplicons representing 20,000 human transcripts in each reaction. This reagent is primarily for transcript counting, not for isomer analysis. “We think this is a great way to measure changes in gene expression in cancer samples because it starts with 10 ng of FFPE DNA,†Felton said. In March of this year, Thermo Fisher also launched Ion Chef, a fully automated instrument that automates the cloning and chip loading of PGM and Proton. According to Felton, the PII chip for Proton is still under development. It is expected to produce between 200 million and 300 million 100-bp reads, suitable for applications such as RNA-Seq. Oxford Nanopore Technologies Thanks to the MinION Trial Program (MAP), users can finally take the U-size MinION sequencer home. Some users have already published their results. Alexander Mikheyev and Mandy Tin of the Okinawa Industrial Technology Center published an article in August, saying that the instrument has low throughput and high error rate. Oxford Nanopore did not respond to this matter. However, computational biologist Keith Robison wrote in his blog: "Mikheyev and Tin failed to explore a variety of parameters or focus on the MAP community, but rushed out of the paper, so their analysis is very Big flaws." At the same time, Oxford Nanopore is also expanding its line of instruments. The newest member is PromethION, a flat-sized benchtop sequencer. It can run a small number of samples in a large number of nanopores or run multiple samples in parallel in thousands of nanopore sensing channels. Pacific Biosciences Last month, Pacific Biosciences announced the release of the latest reagent, called P6-C4 (the 6th generation polymerase and the 4th generation reagent). This new reagent increases the average read length of PacBio to 10,000 to 15,000 bp with a maximum read length of over 40,000 bp. According to reports, the effective data output of each SMRT Cell after the upgrade is expected to reach 500M to 1000M. “By providing a greater amount of long reads each time the instrument is run, the new reagents allow users to assemble the genome with higher quality.†PacBio's long-reading technology is popular in applications such as localized transcript structures and infectious disease surveillance. Recently, some studies have confirmed that this technology is compatible with de novo genome assembly. Third party tool Sequencing vendors are more than just manufacturers that produce sequencers. Swiss automation company Tecan announced the launch of Freedom EVO in September? NGS workstation for library preparation of Illumina and Ion Torrent. According to Tecan, the Freedom EVO NGS workstation provides automation for library preparation, nucleic acid quantification, qPCR system construction, concentration homogenization, sample collection and capture for users without any experience. Agilent Technologies' SureSelect Targeting Sequence Capture Kit has been very popular. In June, it announced the launch of the new SureSelectQXT kit, a revolutionary NGS targeting sequence capture program that produces sequencing-ready libraries from 50 ng of genomic DNA in just 7 hours. Next-generation sequencing has ushered in more and more viewers, and their demand for tools is driving the development of instruments. So, if more new products come out next year, don't be surprised. Source: Biopass Shower Gel,Shower Cream,Shower Foam,Body Shower Gel Wuxi Keni Daily Cosmetics Co.,Ltd , https://www.wxkenidaily.com