We're Professional Supplier Extract Powder manufacturers and suppliers in China specialized in providing high-quality products at low price. We warmly welcome you to buy or wholesale bulk Supplier Extract Powder for sale here from our factory. For a free sample, contact us now.
Supplier Extract Powder,Supplier Extract ,Supplier Powder Manufacturer in China Shaanxi Kang New Pharmaceutical co., Ltd. , https://www.bodybuildingoil.com
The results showed that the editing of CRISPR was specific when DNA was relaxed, but when the DNA was stretched, the editing accuracy of CRISPR was reduced, and off-target editing appeared, and the probability of off-target increased with the increase of force. This research will help design a CRISPR system with higher accuracy.
Hold the molecule & Look at it!
An in-depth understanding of single-molecule interactions helps scientists better answer the most basic life science issues. How to carry out dynamic research of biomolecules at the single molecule level is also a difficult problem in the field of single molecule biophysics. The advent of single-molecule manipulation techniques such as atomic force microscopy and aperture has made this research possible. The C-Trap fluorescence diaphragm system developed by LUMICKS in the Netherlands uses dual aperture + fluorescence confocal imaging technology, which is a powerful tool for studying single molecule interaction! 
The latest surface imaging solution for the LUMICKS Fluorescence System: Surface Assay Toolkit 
Using IRM (interference reflection microscopy) technology, the shape of microtubule microfilaments can be observed in real time without marking, and the background noise is extremely low, which is suitable for dynamic capture of cytoskeleton. In combination with IRM and TIRF, dynamic changes in microfilaments, motor proteins, and other proteins can be observed in real time. 
Combined with surface fluorescence imaging (wide field illumination Widefield and total internal reflection illumination TIRF), biological samples can be quickly imaged for cell-molecule interaction studies. The latest published literature on C-Trap fluorescence spectroscopic system:
The emergence of fluorescent fluorescence systems has enabled scientists to more easily perform a series of single-molecule studies, such as DNA replication, transcription, and protein interactions during translation; conformational changes of small molecules such as proteins; cytoskeleton and intracellular material transport Small molecules interact with proteins.
Salt-Dependent Rheology and Surface Tension of Protein Condensates Using Optical Traps Physical Review Letters, 2018
Molecular Crowding Tunes Material States of Ribonucleoprotein Condensates Biomolecules, 2019
Sites of high local frustration in DNA origami Nature Communication, 2019
DNA stretching induces Cas9 off-target activity Nature Structural Molecular Biology, 2019
Related products and links:
1, Netherlands Lumicks C-Trap super-resolution single-molecule dynamic analyzer (fluorescent diaphragm):
Http://?id=435
2, the Netherlands Lumicks AFS high-throughput molecular manipulation analyzer (sound):
Http://?id=436
3, the Netherlands Lumicks m-Trap ultra high resolution double aperture:
Http://?id=453
4, the Netherlands Lumicks u-Flux laminar flow microfluidic system:
Http://?id=454
Nature Subsidiary: Scientists use the fluorescence pupil system to perform real-time visual assessment of Cas9 off-target effects
Nature Subsidiary: Scientists use the fluorescence pupil system to perform real-time visual assessment of Cas9 off-target effects
As an effective gene editing method, CRISPR-Cas9 has great potential in disease prevention and treatment, but its off-target effect must be considered in clinical transformation. Traditional biological methods such as electrophoresis or sequencing can be used to study the targeting of Cas9, but the results are mostly static and average. The latest article published in the March 3019 issue of the journal Nature Structural and Molecular Biology, using the latest fluorescent aperture technology, using a light-operated DNA template, combined with a confocal fluorescence microscopy imaging system, enables dynamic observation at the single molecule level. Cas9 targeted editing of the gene of interest.
In addition to its use with confocal microscopy systems, LUMICKS recently introduced solutions for IRM, WD & TIRF imaging systems, extending single-molecule research to cytology!