Scallop mushroom culture method

1. Master medium

(1) Maltose yeast agar medium (MYA) 1 g of soybean peptone (soybean meal), 2 g of yeast, 20 g of maltose, 20 g of agar, and water was added to 1000 ml.

(2) Potato Glucose Yeast Agar Medium (PDYA) Potato 300 g (1 500 ml of water, boiled for 20 minutes, using filter juice), 2 g of yeast, 1 g of soybean meal, 10 g of glucose, 20 g of agar, add water to 1000 g Ml.

(3) Oatmeal Maltose yeast agar medium (DMYA) 80 g of oat flour, 10 g of maltose, 2 g of yeast, 20 g of agar, and water was added to 1000 ml.

The above three formulations can be used as a liquid medium without addition of agar. The above medium should be prepared, packed, sterilized, inoculated and cultured as usual.

2. Original and cultivating medium

(1) Wheat straw or bare wheat straw, chopped (2-3 cm long), soaked, bottling, and autoclaved for use.

(2) soak wheat, naked wheat, sorghum, corn, millet and other grains, and cook until no white core but the epidermis is not broken, add 2% calcium carbonate, bottling, and use it after autoclaving.

(3) 40% of sawdust and wood chips, and 20% of bran, and a cultivating medium was prepared.

Can also be used Pleurotus ostreatus or mushroom waste as a medium for re-sterilization after use.

3. Inoculation

It can be inoculated with liquid strains that have been cultured for 3-4 days. If using solid strains must increase the inoculation amount (strain/culture material), the inoculation amount is at least 10%, preferably 15%-20%.

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