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1. Installation and commissioning of CO2 incubator The front of the CO2 incubator has an operating panel with power switch, temperature regulator (manual and liquid crystal display), CO2 injection switch, CO2 adjustment knob, humidity adjustment knob. , temperature display panel, CO2 display panel and humidity display panel, carbon dioxide sample hole (for extracting the sample in the box to detect whether the CO2 in the box reaches the content displayed on the display panel) and alarm device (over temperature alarm light) .
(1) Adjusting the humidity of the CO2 constant temperature incubator First press the humidity adjustment knob, then turn the humidity adjustment knob to the required culture humidity, and then adjust the humidity automatically by the humidity regulator.
(2) Before turning on the power, add a certain amount of distilled water in the incubator according to the instruction manual (add the amount of disinfectant to the water added, see the instructions for details) to avoid burning the machine.
(3) The incubator should be placed in a relatively stable position and away from heat sources to prevent temperature fluctuations and microbial contamination.
(4) The CO2 used in the incubator can use liquid CO2 or gas, no matter which kind of CO2 is used to supply the pipe, the pipe can not be too curved to ensure the smooth flow of gas. Generally, the CO2 cylinder is used, and the pressure control table can be connected.
(5) When the temperature reaches the required temperature, the heating is automatically stopped. When the required temperature is exceeded, the over-temperature alarm light is on and an alarm sounds.
(6) When the water is added to a certain amount, the alarm light is on, that is, the water supply is stopped, and the power switch is turned on, that is, the heating is started, and the temperature controller is adjusted to the required temperature.
(7) Zeroing of CO2 content After the temperature and humidity in the tank are stable (usually three days), turn the CO2 adjustment knob to adjust the number of the display panel to 0.00. Repeat the adjustment after 5 minutes. Show that the reading on the disc is stable. Turn on the CO2 injection switch (injection light is on), adjust the CO2 setting to the desired concentration so that the concentration reaches the set concentration (the injection lamp is extinguished) and maintain it for at least 10 minutes. Thereafter, the CO2 content in the tank is automatically adjusted by the CO2 controller.
Shanghai carbon dioxide incubator features and precautions for use
Shanghai Carbon Dioxide Incubator Features and Precautions for Use The carbon dioxide incubator is improved on the basis of common culture, mainly to add CO2 to meet the environment required for the cultivation of microorganisms. Mainly used for tissue culture and cultivation of some special microorganisms. According to the experimental requirements, the carbon dioxide incubator is divided into water jacket and air jacket.