Recently, some farmers reported that fruit cracking occurred in eggplants; cracking of eggplants did not affect food consumption, but it directly affected the value of eggplant products and caused direct damage to farmers. Causes of eggplant cracking there are many reasons, there are mainly the following three reasons for the reference of farmers. 1. Physiological diseases If flowering, poor flower bud differentiation, easily deformed flowers, leading to fruit deformity or cracking. Such as partial application of high nitrogen fertilizer or spray anther concentration is too high or spraying time too late, there will be fruit deformity or fruit cracking. 2. The soil is dry, and the water supply is not uniform. For example, during the growth period of eggplant, the temperature is high and the air is dry. In the case of flooding in the evening, it is easy to produce cracks. Especially in dry lands that have not been watered for a long time, sudden flooding will increase the water supply of the flesh, and the growth of the peel will be slower, resulting in an inverse relationship between the growth of the flesh and the peel, and more likely to produce cracks. 3. Temperature difference between day and night Eggplant in the growth process, large temperature difference between day and night, crop dry matter accumulation, eggplant fruit growth, and the fruit epidermis affected by the external environment, eggplant in the night when the temperature changes in the case of the so-called "tight hide "phenomenon. This inconsistent growth rate inside and outside, resulting in serious eggplant rip. Control methods 1. Boron fertilizer or boron-containing foliar fertilizer can be sprayed to reduce nutrient imbalance and reduce cracking of eggplant. 2. Watering should be uniform to avoid flooding. 3. Increase warm-keeping measures and try to avoid excessive temperature difference between day and night.
PCR ( Polymerase Chain Reaction)
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It provides a way to make more copies of a portion of DNA.
We need to have PCR ingredients that include DNA portion, Buffer solution, Primer+DNA (tap polymerase), and Nucleotides during the preparation step.
In the step of PCR sequence, there are three steps in this section.
Step 1 Denaturation
The addition of heat is needed to separate the two strands of the DNA molecule.
Step 2 Annealing
Temperature, is going to be cool in this step, should allow the primers to bind to the specific segments.
Step 3 DNA Synthesis
To make more copies of the DNA.
Those three steps above keep repeating to make enough copies of the DNA fragments. It is the principle of how PCR machine works.