Our organic vegetable powder mainly used dehydration technology. Dehydration vegetable powder remained most fiber and taste of fresh vegetables, so they are suitable to be added in sauce, stuff, pudding, yogurt and dessert. Some vegetable powder could be applied as pigment like red beet root powder. Some could be mixed with other super green powders to make health formula, kale powder and broccoli powder are always good choice for customers.
Vegetable Powder,Red Beet Root Powder,Broccoli Powder,Organic Vegetable Powder YT(Xi'an) Biochem Co., Ltd. , https://www.ytwholefood.com
TA clone common problems and solutions
The TA cloning kit (TA) is a method in which a PCR fragment is ligated to a vector DNA having a 3-T overhang. Since the polymerase used in the PCR reaction has terminal transfer activity, it is usually 3 plus A. For example, Taq polymerase has the function of a terminal ligase, and a 3-A overhang is automatically added to the 3 ends of each PCR amplification product in the PCR reaction. Only a specially processed DNA fragment with a 3-T overhang can be ligated by T/A pairing. In the process of amplifying a target gene fragment by a PCR method, since the DNA sequence of the target gene is often unclear, the obtained target fragment usually needs to be recombined into a T-vector by TA cloning, and the DNA sequence is clearly determined by sequence determination.
Because the DNA polymerase used in the PCR process is different, it is often divided into two cases: (1) using different Taq enzymes, after the end of the PCR amplification cycle, adding a process of 72 ° C for 10 minutes, the Taq enzyme can be The 3 terminus of the amplified product is added with A, so that the PCR product can be directly linked to the T vector after recovery and purification. (2) Using a high-fidelity DNA polymerase, such as a pfu enzyme, since it cannot add A to the 3rd end of the amplified product, the obtained DNA sequence is blunt-end, and therefore, it is necessary to carry out the process of adding A after recovery and purification. Usually, the PCR product is used as a template, a certain amount of ordinary Taq enzyme and reaction solution are added, dATP (or dNTP) is added, and the product is added to the TA for direct use at 72 ° C for 10 minutes.
TA clone common problems and solutions