The ultra-micro spectrophotometer is a precision optical instrument. It has been carefully assembled and debugged before leaving the factory. If the instrument can be properly maintained and maintained, it can not only ensure the reliability and stability of the instrument, but also prolong the service life of the instrument. (1) Requirements for the working environment of the instrument <br> The requirements for the working environment of the ultra-micro spectrophotometer are as follows. Robot Calibration Solution Robot Calibration Automated Precision Inc. , https://www.apiasean.com
Ultra-micro spectrophotometers are commonly used to quantify oligonucleotides, single-stranded, double-stranded DNA, and RNA in buffer. The absorption peak of the highest absorption peak of nucleic acid is 260 nm. The molecular composition of each nucleic acid is different, so the conversion factor is different. To quantify different types of nucleic acids, select the corresponding coefficients in advance. For example, the absorbance of 1 OD corresponds to 50 μg/ml of dsDNA, 37 μg/ml of ssDNA, 40 μg/ml of RNA, and 30 μg/ml of Olig. The absorbance after the test is converted by the above coefficients to obtain the corresponding sample concentration.
The working principle of the ultra-micro spectrophotometer is an instrument for quantitative and qualitative analysis of substances by spectrophotometry, which is commonly used for nucleic acid, protein quantification and quantification of bacterial growth concentration. Whether in the fields of physics, chemistry, biology, medicine, materials science, environmental science, or modern production and management in chemical, pharmaceutical, environmental testing, metallurgy, etc., UV-visible spectrophotometers are widely and importantly application.
The ultra-micro spectrophotometer is a precision optical instrument. Proper installation, use and maintenance are important for maintaining good performance and ensuring the accuracy of the test. Let's share some of the general knowledge of ultra-micro spectrophotometers.
1 The instrument should be placed in a dry room at a temperature of 5 to 35 ° C. The relative humidity does not exceed 85%.
2 The instrument should be placed on a firm and stable work surface and avoid strong vibration or continuous vibration.
3 indoor lighting should not be too strong, and should avoid direct sunlight.
4 The electric fan should not be directly blown to the instrument to prevent the light source lamp from affecting the normal use of the instrument due to unstable illumination.
5 Try to stay away from high-intensity magnetic fields, electric fields, and electrical equipment that generate high-frequency waves.
6 The power supply voltage supplied to the instrument is AC 220V±22V, the frequency is 50Hz±1Hz, and a good grounding wire must be installed. It is recommended to use an electronic AC voltage regulator with an electric power of 1000w or more or an AC constant voltage regulator to enhance the anti-jamming performance of the instrument.
7 Avoid use in places where corrosive gases such as hydrogen sulfide are present.
(2) Daily maintenance and maintenance of the instrument 1 light source. The life of the light source is limited. In order to prolong the service life of the light source, do not turn on the light source when the instrument is not in use. The number of switching should be minimized. The lights may not be turned off during short working intervals. The light source that has just been turned off cannot be turned back on immediately.
The continuous use of the instrument should not exceed 3 hours. If you need to use it for a long time, it is best to pause for 30 minutes. If the brightness of the light source lamp is significantly weakened or unstable, the new lamp should be replaced in time. After the replacement, adjust the position of the filament. Do not touch the window or the bulb directly with your hands to avoid oil stains. If you have accidentally touched it, wipe it with absolute ethanol.
2 monochromators. The monochromator is the core part of the instrument and is housed in a sealed box and cannot be disassembled. The selected wavelength should be rotated in a balanced manner, and the unavailability is too strong. In order to prevent the dispersive elements from being exposed to moisture, the monochromator desiccant (silica gel) must be replaced periodically. If the desiccant is found to be discolored, it should be replaced immediately.
3 absorption pool. The absorption cell must be used correctly and special care should be taken to protect the two optical faces of the absorption cell.
4 detectors. The photoelectric conversion element cannot be exposed for a long time, and strong light irradiation or moisture accumulation should be avoided.
5 When the instrument stops working, the power must be turned off.
6 In order to avoid dust and contamination of the instrument, the dust cover should be covered when the work is stopped.
7 If the instrument does not need to be energized regularly, it should be no less than 20~30min each time to keep the whole machine dry and maintain the performance of electronic components.