Aflatoxin B1 immunoaffinity column ( AFB1-IAC ) 1. Product introduction Aflatoxin B1 (AFB1) is a carcinogenic and mutagenic substance and belongs to one of aflatoxins. It poses a great threat to human health. Therefore, all countries have strict aflatoxin B1 (AFB1) content in food. Limited standard. This product cross-links a specific aflatoxin B1 (AFB1) monoclonal antibody on agarose gel microspheres. Based on antigen-antibody specific binding, the sample is purified and aflatoxin B1 (AFB1) specific. Sexual binding on the immunoaffinity column, other substances flow out, after leaching, elution, collection, detection, etc., complete the aflatoxin in the sample Determination of B1 (AFB1). The efficiency, recovery rate and maximum load of this product are in line with national standards: GB 5009.22-2016 (Determination of aflatoxin B and G in foods in national food safety standards) GB/T 18979-2003 (aflatoxin determination in food - immunoaffinity chromatography purification high performance liquid chromatography and fluorescence spectrophotometry). 2. Use It is suitable for the determination of aflatoxin B1 (AFB1) in foods (such as corn, peanuts and their products, rice, wheat, vegetable oil, soy sauce, vinegar, etc.); purification and extraction of aflatoxin B1 (AFB1) in samples Wait. 3. Product performance performance index Matrix Agarose microspheres Ligand Aflatoxin B1 monoclonal antibody Load AFT B1 ≥ 300ng / support Recovery rate ≥85% specification 3ml*25 sticks/set Particle size 45~165μm Load recommended flow rate 1-2 drops / sec Elution recommended flow rate 2-3 seconds / drop Operating temperature Room temperature (about 22-25 ° C) Storage buffer PBS buffer Storage temperature 4-8 ° C (not allowed to store below 0 ° C) Validity period 12 months 4. Operation process 1 Sample treatment: The samples were treated according to the respective methods in GB 5009.22-2016 (Determination of aflatoxin B and G in the food safety national standard food). 2 Purification: Take GB 5009.22-2016 (Determination of aflatoxin B and G in food safety national food as an example) (1) Column pretreatment: remove the aflatoxin B1 immunoaffinity column, return to room temperature (22-25 ° C), remove the upper end plug, open the lower end plug, and drain the column preservation solution to a higher gel interface. At 3-5 mm, stop fluid flow (or connect the immunochromatographic column directly to a 20 ml syringe). (2) Adding sample: Add the processed sample liquid to the column, open the lower end plug, adjust the flow rate to support 1-2 drops/second, and discard the effluent. Note: rice, corn, wheat, peanuts and their products, vegetable oil: add 15ml of soy sauce, vinegar: add 10ml of processed sample (2) Rinsing of rice, corn, wheat, peanuts and their products, and vegetable oil samples: 10ml after the liquid is completely drained Distilled water, rinse twice, discard the effluent. Soy sauce and vinegar samples were rinsed: After the liquid was completely drained, wash it with 10 mL of 0.1% Tween-20 in PBS, discard the effluent; rinse with 10 ml of distilled water twice, discard the effluent. (4) Blow dry: Use vacuum equipment (such as suction balls, syringes, etc.), press in the air, and dry the water in the column. (5) Elution: Add 1 ml of methanol (chromatographic grade), adjust the flow rate to 2-3 seconds per drop, slowly elute, and collect all the eluate (ie, the purified sample). 3 detection The purified sample can be directly detected by ELISA, fluorescence and high performance liquid phase. 5. Precautions 1 Aflatoxin B1 is a carcinogen and should be avoided in direct contact with the body. Please wear gloves, masks, lab coats, etc. when handling. 2 The glass container that has been used in contact with the experiment should be soaked overnight with 5% sodium hypochlorite solution to avoid contamination. 3 Transportation: 4-25 ° C, atmospheric pressure, protected from light. SARMs is a new performance-enhancing compound that is shaking up the fitness industry. They have the ability to promote muscle growth, fat loss, and even cardiovascular endurance. To maintain legitimacy, these new compounds, sold for "research purposes," are emerging as promising alternatives to anabolic Steroids. A lot of people are trying to promote dramatic changes in SARM's body structure. sarms for sale,buy sarms online,sarms side effects,sarms supplement,sarms bodybuilding Shaanxi YXchuang Biotechnology Co., Ltd , https://www.peptidenootropic.com
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