chemical composition The fruit contains flavonoids such as hawthorn phenol, kaempferol 1-3 glucoside, kaempferol 1-3 rutose glycosides, tinea quinone (Tribuloside), luteolin, and rutin; as well as diosgenin and sea cucumber. Saponins, Furostanol glycoside saponinC, and 3-Deoxy-△3-diosgenin and other steroidal saponins, of which diosgenin is the main component. The seeds contain the alkaloids Harman and Hatmine. 1. Reagents and instrument reagents are pure, analytically pure. UNIFY? 400 nuclear magnetic resonance apparatus; LCQ? 1700 type electrospray mass spectrometer; Perkin? Elmer 241 type polarimeter; WATERS? 600E high performance liquid chromatograph, the preparation column is SHIM?PACK PREF?ODS. 2. Extract and isolate the total homemade saponins (20 g), dissolve in methanol, and chromatograph on silica gel, eluting with a gradient of CHCl 3 :MeOH:H 2 O, to obtain A, B, and C fractions. The fraction B was separated by silica gel column chromatography and eluted with a gradient of CHCl 3 :MeOH:H 2 O. The eluted fractions from 111 to 130 were separated by HPLC to give compounds I, II and III. 3. Thin layer acid hydrolysis of saponins Take a small amount of compound I, II, and III, respectively, dissolve in methanol, place on high-efficiency thin layer plate, place concentrated hydrochloric acid in steam for 12 hours, dry concentrated hydrochloric acid, use standard sugar, use chloroform: methanol. : Water unfolds and develops with aniline phthalate. Frozen Bonito Skipjack Whole Round Zhoushan City Shuangying Aquatic Products Co., Ltd.  , https://www.shuangying-aquatic.com