product name Crystal violet staining solution Item number JM0602 specification 100ml Storage Conditions Keep away from light at room temperature product content: 1. Crystal Violet Staining Solution (Crystal Violet Staining Solution) is a staining solution commonly used in tissue or cell staining to stain the nucleus into dark purple. 2. Crystal violet is a basic dye that binds to DNA in the nucleus to produce nuclear staining. 3. A package of this stain can stain at least 200 samples. 4. Store at room temperature and protect from light, valid for one year. Instructions for use: 1. Sample Preparation a) For paraffin sections: dewaxed in xylene for 5-10 minutes. Switch to fresh xylene and dewax for 5-10 minutes. Anhydrous ethanol for 5 minutes. 90% ethanol for 2 minutes. 70% ethanol for 2 minutes. Distilled water for 2 minutes. b) For frozen sections: distilled water for 2 minutes. c) For cultured cells: fixed with 4% paraformaldehyde for more than 10 minutes. Wash with distilled water for 2 minutes. Switch to fresh distilled water and wash for another 2 minutes. 2. Crystal violet staining For the above-prepared sample: Crystal violet staining solution was dyed for 10 minutes (the time can be adjusted according to the staining results and requirements). After washing with distilled water or tap water, it can be observed and photographed. Precautions: 1. You need to bring your own 4% paraformaldehyde. If you need dehydration, transparency and sealing treatment, you need to bring your own xylene, neutral gum or other sealing tablets. If the sample is paraffin section, you need to bring your own 70% and 90% ethanol, absolute ethanol and xylene. 2. When the number of samples is large, the dyeing rack and dyeing tank can be used for easy operation. 3. When using this kit for the first time, it is recommended to take 1-2 samples for pre-experiment. 4. Crystal violet is harmful to the human body, please pay attention to proper protection. 5. For your safety and health, wear a lab coat and disposable gloves. Validity: Valid for 12 months. If you encounter any technical problems in the experiment, please consult the company's technical staff in time. Air Dryer,Desiccant Air Dryer,Industrial Hot Air Dryer Machine,Industrial Air Dryer Hangzhou Zhongju air separation equipment manufacturing Co., Ltd , https://www.hzzjkfzz.com