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Daily maintenance and maintenance of pipettes
Pipettes are one of the most commonly used instruments in the laboratory and one of the instruments most likely to affect the results of the experiment. In order to ensure the pipetting accuracy of the pipette and ensure the accuracy of the experiment, when using a pipette to encounter common faults, How to operate and solve the pipette failure has a great effect on improving the efficiency and accuracy of the experiment. Here, Laibei would like to talk about the daily maintenance and maintenance of the pipette in this article, hoping to extend the life of the pipette and improve the efficiency and accuracy of the experiment.
1. If not used, adjust the pipette's range to the maximum value to keep the spring in a relaxed state to protect the spring.
Second, it is best to clean the pipette regularly. It can be washed with soapy water or 60% isopropanol, distilled water, and dried naturally.
Third, before high temperature sterilization, to ensure that the pipette can adapt to high temperatures.
Fourth, the calibration can be carried out in a 20-25 degree environment by repeating the method of weighing distilled water several times.
5. When using the pipette, check for leaks. The method is to take the liquid and then hang it vertically for a few seconds to see if the liquid level drops. If the pipette leaks, there are roughly the following reasons:
1. Whether the pipetting gun matches;
2. Whether the pipette spring piston is normal;
3. If it is a volatile liquid (as is the case with many organic solvents), it may be a problem of saturated vapor pressure. You can suck up the liquid several times before pipetting.
In fact, in addition to the above daily maintenance and maintenance of the pipette can improve the accuracy of the experiment, if you can choose a pipette with high accuracy and accuracy, it is also an important factor. Improve the efficiency and accuracy of the experiment.
Just like the high-quality pipettes from the two series of IKA pipettes ALPHA and IKA pipettes PRECISION, these two series of IKA pipettes are set for accuracy, accuracy, safety and hygiene. Industry standards to provide an enhanced pipetting experience. IKA pipettes are available in both fixed and adjustable ranges; single-channel IKA pipettes have a range of (0.1 to 10,000 μl) and multi-channel IKA pipettes have a range of (0.5 to 300 μl) ) to meet the diverse needs of customers. The user can calibrate the pipette by himself using the proprietary IKA pipette calibration software.