The eStain L1 Protein Stainer is a highly efficient protein staining system that uses proprietary protein staining technology developed by Kingsray Biotech. eStain L1 Rapid Electrostaining combines the traditional immobilization-staining-decolorization three-step reaction to achieve stable, efficient, fast and reliable protein on polyacrylamide gels in 10 minutes or less. Blue staining. No additional auxiliary reagents are required for the entire dyeing and decolorization process, and the dyeing efficiency and sensitivity are high. The following is a description of Coomassie Brilliant Blue rapid staining of small gels using the eStain L1 Protein Stainer. For detailed instructions, please read the eStain L1 Protein Stainer User Manual: Dyeing specific operations: Step 1. When your gel electrophoresis is almost finished, turn on the switch on the back of the eStain L1 protein stainer, and the instrument beeps. At this time, the instrument performs a power-on self-test. After the self-test is finished, the main screen lights up. The main interface is as follows: Figure. Step 2. Inject a proper amount of distilled water into the tray to completely infiltrate the gel. Step 3. Remove the gel retaining clip from the instrument, place the infiltrated gel horizontally on the mesh surface of the gel retaining clip, and then lay the monolithic filter paper infiltrated in the tray on the gel. Close the gel clip. Step 4. Insert the gel clip into one of the channels of the instrument in a directional orientation (as shown in the figure below, the gel clip is inserted into the B channel of the instrument) Step 5. Press the START B button at the bottom of the main screen, the instrument will start to dye, and the main interface will display as shown below. At this time, the START B button will flash and the dyeing will start counting down. After the instrument is finished, the dyeing time is 0, the instrument beeps, press the START B button, the button flashes to stop, and the main interface returns to the following interface. Step 6. After the dyeing is completed, the gel fixing clip can be taken out, and the gel is sequentially taken out. eStain L1 Protein Stainer Operation Video: Rice Seeding Machine,Rice Seeder Machine,Rice Seeder Farm Equipment,Rice Seed Sowing Machine Taizhou Yingtian Agricultural Machinery Manufacturing Co., Ltd. , https://www.sakuradaagc.com
Note: The gel needs to be infiltrated in this tray for about 1 minute. If you try the Life Tech. brand gel GEL, you must remove the raised parts.
Note: * One end of the inner surface of the fixed clip is tiled horizontally. Refer to the figure below to ensure that the gel is placed in the same direction and as close as possible to the center axis when the gel does not exceed the net amount. 
Note: When installing the gel fixing clip, it should be noted that the one with the mesh surface is facing the operator, and then the gel fixing clip is inserted vertically into the channel groove. If the gel fixing clip is inserted in the wrong direction, the instrument may be damaged. 
