Masson Trichrome Staining Kit Instruction Manual

product content:

1. (Masson) dyeing solution is one of the main methods to show fiber dyeing in tissues. It is the authoritative and classical technical method for collagen fiber dyeing. Mainly used for the differential analysis of collagen and smooth muscle. Widely used in the study of connective tissue, muscle tissue and collagen.

2. Masson trichrome staining kit stained collagen fibers, mucus, cartilage in blue (such as light green staining green); cytoplasm, muscle, cellulose, glial red; nucleus blue-violet.

3. It is non-toxic, environmentally friendly, harmless to the human body, simple in operation, stable in performance, clear in color, bright in color, clear in contrast and stable in dyeing, long in storage time and not easy to fade, etc. Performance, dyeing of anionic dyes with different molecular sizes can be used to color different tissue components.

4. Packing list:

5. Store at room temperature and protected from light for at least one year.

Instructions for use:

1. Paraffin sections are dewaxed to water;

2. Chromating treatment or removal of mercury salt precipitation (formaldehyde fixed tissue can be omitted);

3. Wash tap water and distilled water in turn;

4. Dye the core with reagent A for 5-15 min;

5. Fully washed, blue after microscopic examination, until the nucleus is blue, such as over-stained hydrochloric acid alcohol differentiation;

6. Wash with distilled water; dry the water on the slide, place the slide in a 65-degree oven or a 37-degree incubator for a few minutes until the surface of the slide has no moisture, and the surface of the specimen is dry and whitish;

7. Place the dried slides horizontally on the immunohistochemical wet box. For laboratories without this box, it is recommended to place the slides horizontally on the tray or in any container you can find. On the specimen, add a few drops of reagent B, and cover the specimen with the dye solution. Place horizontally, stain at room temperature for 10-20 min;

8. Using reagent C, clean the slide and remove the dye B for 3 minutes, 3 times each time;

9. The water on the dried glass slide is diluted with the aqueous solution of reagent D for 3-5 minutes;

10. Filter out reagent D with filter paper, taking care not to touch the sample with the filter paper. Without washing with water, directly dye with reagent E for 5-15 seconds;

11. Wash the slide with the reagent C solution to remove the excess dye solution, so that the dyed color is clear and vivid;

12. 95% alcohol, absolute alcohol, xylene transparent, neutral gum seal.

Precautions:

1. 95% alcohol, absolute alcohol, xylene transparent, neutral gum should be prepared.

2. There are many operating procedures in this method. It is necessary to control the dyeing steps. After mastering, you can get satisfactory dyeing results.

3. Tissue fixation plays an important role in lengthening or shortening the dyeing time depending on the fixing solution;

4. The reagent C is washed with 0.2% glacial acetic acid aqueous solution to make the color tone clear and vivid;

5. Reagent D has a differentiation effect on Reagent B. When it is differentiated, it is controlled under the microscope, the muscle fiber and cellulose are red, and the collagen fiber is light pink.

6. For your safety and health, wear a lab coat and disposable gloves.

Validity: Valid for 12 months.

If you encounter any technical problems in the experiment, please consult the company's technical staff in time.