QM100 tissue grinder grinding corn leaf method purpose: Mass milling of corn leaves provides convenience for subsequent extraction of nucleic acids for genetic or physiological biochemical analysis. For the seed and agricultural product quality management department, seed companies and other units to use molecular methods to detect sample purity, detect the authenticity of purity and other sample pre-processing work necessary for reference. Location: A certain industry breeding and cultivation laboratory Material: fresh corn seedling leaves Instrument: Main unit, QM100 tissue grinder; Accessories: 2ml grinding adapter, 3 or 5mm diameter grinding beads step: 1) Sampling: Take the leaves of fresh corn seedlings and cut them into pieces of 6~8mm in diameter; 2) Place 3-5 leaf pieces in a 2 ml centrifuge tube (EP tube) while adding 1 5 mm and 2 3 mm stainless steel ground steel balls. 3) Seal the EP tube with the blade and the grinding steel ball and put it into the 2ml grinding adapter of the QM100 tissue grinder for grinding the corn leaf method, then freeze it with the adapter into the liquid nitrogen for 2-3 minutes or until the liquid nitrogen does not " Boiling". 4) quickly remove the adapter from the liquid nitrogen and quickly fix it in the rocker arm of the grinder; 5) The grinding time of the method of setting the QM100 tissue grinder for grinding corn leaves was 2 minutes, and the grinding frequency was 1500 rpm (1500 times/min). result: Very finely divided leaf powder can be obtained, and the extraction of nucleic acids works well. in conclusion: Using the QM100 tissue grinder to grind the corn leaves. The corn leaves are frozen and ground. The grinding tube and adapter can be directly immersed in liquid nitrogen. It takes only 1-2 minutes of liquid nitrogen freezing and about 2 minutes of grinding. Up to 54 samples of 2.0 ml can be pulverized in batches (192 samples with 96-well deep well plates). The treatment time is fast, effectively preventing the temperature rise, and providing a guarantee for the subsequent effective early nucleic acid. In addition, it also saves a lot of liquid nitrogen and is safer to operate. QM100 tissue grinder grinding corn leaf method Barrier Mesh,Plastic Fence Mesh,Insect Barrier Netting,Agfabric Insect Netting Changzhou Satidi Import and Export Co., Ltd. , https://www.guanjiejts.com