First, the preservation of liquid paraffin liquid paraffin autoclaved, placed in a thermostat for several hours, or placed in a desiccator for several days, with a sterile pipette or syringe to suck liquid paraffin, into the bacteria test tube. The injection volume should be about 1 cm above the beveled tip. Plug the tampon and seal it with wax. Store it in an upright position and keep it for 5 years. Second, bran preservation method Weigh a certain amount of bran, add water or nutrient solution and mix well to use. The wet bran was placed in a test tube, autoclaved and then inserted into the strain, and cultured at a suitable temperature. After the mycelium has developed, the test tube is dried at room temperature. After drying, it shall be stored in a dry place below 20°C and stored for 3 to 5 years. Third, the preservation of physiological saline bacteria will be inserted into the culture solution of the potato, with a 250 ml flask containing 60 ml of the culture medium, shake culture or shake 5 to 10 times a day, culture for 5 to 7 days, and then the formation of the mycelium ball Transfer to a test tube containing 5 ml of sterile physiological saline, transfer 4 to 5 hyphal balls per tube, stuff cotton eggs, and seal the tube with wax. At low temperatures, it can be stored for 1 year. Fourth, the wheat preservation method to take high-quality wheat, washing after soaking in 20 °C water for 5 hours, a little dry, tube. The loading amount is preferably 1/4 to 1/3 of the pipe length. Sterilization, heat and shaking, cooling access to mycelium. Incubate at appropriate temperature until the sparse hyphae appear on most grains. Then, place it in a dry place below 25°C and store it for 1 to 2 years.
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