How to prevent three pests in spring

Before the budding of the apple tree in mid-to-late mid-March, the whole tree sprayed 3-5 Baume degrees lime sulfur mixture, such as Yang Bai Lun tussock moth, need to spray a pyrethroid agent, when the lobular disease serious orchard spraying should be added 1 % of zinc sulfate. Scrape cure branch rot disease, ring disease, scrape after the coating of 9281, 843 rehabilitation agent, antzovirus poisoning, rot will be clear, rot will cure, Fuxing, Guo Kang Bao and other agents. Scrape should be light should not be heavy to prevent the tree from weakening caused by excessive weight. In April, for new Red Star, Red Star, Marshal, and Beidou varieties of susceptibility to fungal disease, and in previous years, the red orchard fibrous disease orchard was heavy, they each sprayed a 1000-fold multi-effector or 1000-fold each at the early flowering stage and late flowering stage. Liquid thiophanate-methyl, or 500-fold solution of 1.5% polyoxin or 800-times 3% guankang 120, is effective for prevention.

Pyrus sprouts before spraying 3-5 Baume degree lime sulfur agent, the big trees should pay attention to spray to big branches, big stems and old chapped skin. Pear buds exposed when sprayed 1500 times liquid beta cypermethrin control pear hibiscus, pear borer, pear bifurcation, yellow-flies. The pear hibiscus spawns eggs in high temperature, no-wind weather in late March, and 1500 times liquid cypermethrin + 1500 times phoxim in the whitening period kill eggs. This medicine is very important. When 80% is dropped, spray 50% carbendazim powder 600-800 times liquid plus fleas 1500-2000 times to control pear black spot disease, pear hibiscus, pear bifurcation and other pests. This period is a critical period for the prevention and treatment of pear bifurcation. Imidacloprid drugs must be added during spraying. At the same time, attention should be paid to the prevention and treatment of pear stem bees. One is to spray pyrethrum pesticides, and the other is to manually remove the harm of pear stem bee.

Before the germination of the peach trees, 3-5 Baume degree lime sulfur agent was used to control peach aphid, red spider, bacterial perforation, and scale insects. Sprayed once before 3000-4000 times liquid 10% imidacloprid in peach. After spraying, spray one time 3000-4000 times liquid 10% imidacloprid or pyrethroids plus 600-800 times 50% carbendazim powder or add 50% epimedium 600 times and add 300 times urea to prevent and control peach aphid , leaf disease, bacterial perforation. Before and after the flowering period, the adults of P. rapae adults were active. When spraying imidacloprid after flowering, 1500 times pyrethroid pesticides should be added.

Nucleic Acid (DNA/RNA) Extraction Kit

1. Introduction

The total viral nucleic acid extraction kit is suitable for extracting total viral nucleic acid from serum, plasma, tissue homogenate and other samples. The kit is based on silica column purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. This product has successfully extracted nucleic acids from hepatitis B A/C, hepatitis C, and norovirus standard. The obtained DNA/RNA can be directly used in a series of downstream experiments such as PCR, RT-PCR, and LAMP.
Notice:

1. The carrier RNA solid must be dissolved in Nuclease Free Water to 1µg/µl before use, and vortex to dissolve. Store in aliquots at -70°C. If you need to store it at -20℃ for a long time, please repackage it according to the number of times of use.

2. Dissolve Proteinase K (20mg/ml): Add Proteinase Dissolve Buffer to dissolve Proteinase K to a final concentration of 20mg/ml. Proteinase K dry powder can be stored at 2-8°C for one year, but dissolved Proteinase K must be stored in aliquots at -20°C. Repeated freezing and thawing of Proteinase K can affect its activity.

3. Buffer VHB must be diluted with 14 ml absolute ethanol before use and stored at room temperature.

4. Buffer RW2 must be diluted with 80 ml of absolute ethanol before use and stored at room temperature.

3. Shelf life

Except for Proteinase K and Carrier RNA, other components of this product can be stored at room temperature (15-25°C) for 12 months, and should be stored at 2-8°C for long-term storage. Proteinase K and Carrier RNA dry powder are transported at room temperature. Please store at -20°C after receiving the test product, and store at -20°C after dissolving.

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