Turn on the power switch (lower left corner of the rear panel of the chassis), and the screen displays “Xinzhi Brandâ€. Press ( ), to the transducer selection interface, as shown in Figure 4 (if you do not press ( ), the screen will stay on the startup interface) From the startup interface to the transducer selection interface, you must first select the model of the horn. That is, the X in the horn: ΦX in Figure 4 will continue to jump. Please select the horn that corresponds to your installation. Model (The horn type available for the company is: Φ2, Φ3, Φ6, Φ8, Φ10, Φ15, 6 specifications) The size of the horn is selected by the number keys on the keyboard. After selecting, press SET or ENTER to enter the working interface. parameter settings Press the SET button to enter the parameter settings. After entering, use (â–²) or (â–¼) to select different setting items. The last digit of a certain setting value will continuously jump, indicating that the setting state of the item has been entered. ·Ultrasonic on time setting Use (â–²) or (â–¼) to select the ultrasound on time setting item (“first jump of “0.1sâ€), just type the number you want. ·Ultrasonic off time setting Use (â–²) or (â–¼) to select the ultrasonic off time setting item (the first beat of “ultrasonic off 1.0sâ€), just type the required number. ·Slot temperature setting Use (â–²) or (â–¼) to select the slot temperature setting item (set the first jump of “30°C†above the thermometer in the interface), just type the required number. ·Ultrasonic output power setting Use (â–²) or (â–¼) to select the ultrasonic output power setting item (set the first jump of “10%†below the power output bar in the interface), just type the required number.
The RNA purification kit is used to purify and recover RNA molecules transcribed in vitro and total RNA extracted from various materials, which can effectively remove contaminating impurities in RNA samples. The recovery rate of this product can reach 80%, and the OD260/OD280 ratio of the obtained RNA is generally about 2.0, which can be directly used in subsequent sensitive experiments (such as microarray analysis, fluorescence RT-PCR, etc.). With the deepening of transcriptomics, the complexity of RNA types, expression regulation and functions is far beyond our imagination. Covid-19 Nucleic Acid Extraction Reagent,Nucleic Acid Extraction Reagent Kits,Dna Purification Kit,Rna Purification Kit Jilin Sinoscience Technology Co. LTD , https://www.jlgkscience.com
Removing ribosomal RNAs that account for more than 80% helps to focus sequencing on less abundant but informative RNAs. At present, the removal of rRNA is mainly through the combined use of probe and RNase H. The processed RNA will be mixed with many digestion products, enzymes and ions, which is not conducive to the subsequent construction of RNA library. Take the Columnar RNA Purification Kit as an example. Trizol is a ready-to-use reagent that can be used to purify total RNA from tissues and cells. This is a single-phase solution of phenol and guanidine isothiocyanate that facilitates lysis of tissues and cells, inhibiting RNases to maintain RNA integrity.